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The common influenza ELISA kits are solid phase sandwich ELISA ( Enzyme-Link Immunosorbent Assay), which are to be used for the in-vitro quantitative determination of hemagglutinin with high sensitivity and specificity. 0000144544 00000 n A fourth ELISA test does not use the traditional wells.
This antigen will be recognized and bound by a detection antibody conjugated to biotin and streptavidin-HRP.
0000055055 00000 n However, the Western blot is no longer used, and today the ELISA test is followed by an HIV differentiation assay to confirm HIV infection. The less antigen in the sample, the more labeled antigen is retained in the well and the stronger the signal. 0000172225 00000 n 0000171743 00000 n 0000083111 00000 n 0000125500 00000 n
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0000159251 00000 n Pipettes, washer system, ELISA plate reader: Readers, washers and pipette are available as manual or automated system. 0000203878 00000 n 0000042614 00000 n 0000073275 00000 n 0000175866 00000 n 0000150718 00000 n <<8A8DD9EF8DFFA945AD87D72D860EF527>]/Prev 3799105/XRefStm 18181>>
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A specially prepared … 0000177485 00000 n 0000142103 00000 n
0000060746 00000 n 0000130824 00000 n 0000042364 00000 n 0000094403 00000 n 0000049287 00000 n 0000141290 00000 n 0000126792 00000 n 0000191279 00000 n 0000104906 00000 n 0000079454 00000 n 0000113464 00000 n 0000158846 00000 n If only one is present, it is not. 0000189244 00000 n I.e.
0000144216 00000 n 0000121533 00000 n 0000100147 00000 n
0000112170 00000 n 0000136827 00000 n 0000185914 00000 n 0000188025 00000 n 0000090420 00000 n 0000202256 00000 n 0000094809 00000 n 0000154294 00000 n 0000103370 00000 n
0000077423 00000 n 0000191686 00000 n 0000119514 00000 n 0000086684 00000 n 0000115400 00000 n
0000175461 00000 n 0000185506 00000 n
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ELISA Readers: Readers need to have appropriate filter (650 nm and 450 nm).
0000142996 00000 n However, to get the test results, it may take several days, and in some cases weeks.
0000124772 00000 n 0000205505 00000 n 0000170607 00000 n 0000074903 00000 n 0000159172 00000 n A medical professional will:To decrease further bleeding, after the test the person may be asked to elevate or flex their arm to reduce blood flow. 0000141775 00000 n 0000105791 00000 n
0000189573 00000 n 0000082218 00000 n 0000186398 00000 n If antibodies to HIV are present in the serum, they may bind to these HIV antigens. 0000025412 00000 n 0000163224 00000 n 0000113385 00000 n 0000158439 00000 n 0000162899 00000 n 0000099661 00000 n 0000192498 00000 n 0000146657 00000 n An example of a competition ELISA to test for antigen based on the direct detection method is shown in Figure 5 .
0000206725 00000 n 0000192014 00000 n 0000144950 00000 n If both tags are present on a cell, then the cell is that specific strain. 0000183070 00000 n 0000190466 00000 n No antigen is left for the enzyme-labelled specific HIV antibodies. 0000184369 00000 n 0000061560 00000 n 0000119435 00000 n
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To test for HIV, a series of blood screenings may be done, including one called the ELISA test. 0000166470 00000 n 0000108615 00000 n
Two or three times the standard deviation (error inherent in a test) is often used to distinguish positive from negative samples.
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ELISA is so named because the test technique involves the use of an enzyme system and immunosorbent.
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Read on to learn how these tests are done, what to expect during the tests, and what the results can mean.The ELISA test is typically the first test ordered by a healthcare provider.
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Qualitative results provide a simple positive or negative result (yes or no) for a sample.
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